Fast 3D super-resolution fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
A guide to super-resolution fluorescence microscopy
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new ap...
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THE ROYAL SWEDISH ACADEMY OF SCIENCES has as its aim to promote the sciences and strengthen their influence in society.
متن کاملReview of super-resolution fluorescence microscopy for biology.
Several methodologies have been developed over the past several years for super-resolution fluorescence microscopy including saturated structured-illumination microscopy (SSIM), stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM), fluorescence photoactivation localization microscopy (FPALM), and stochastic optical reconstruction microscopy (STORM). Whi...
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Motion analysis plays an important role in studing activities or behaviors of live objects in medicine, biotechnology, chemistry, physics, spectroscopy, nanotechnology, enzymology, and biological engineering. This paper briefly reviews the developments in this area mostly in the recent three years, especially for cellular analysis in fluorescence microscopy. The topic has received much attentio...
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Fluorescence microscopy is currently one of the most powerful and versatile techniques available for biological studies. Fluorophore-labeled molecules are very bright and readily distinguishable from other background signals, making it easy to obtain high-contrast images. With the development of genetically encoded fluorescent proteins, it has become possible to image protein expression, locali...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2010
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.f.335